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SRX20809121: Tm and TAdm co-culture genome sequencing
1 ILLUMINA (Illumina HiSeq 2500) run: 5.3M spots, 1.6G bases, 488.1Mb downloads

Design: genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified by AMPure XP system (Beckman Coulter, Beverly, USA), DNA concentration was measured by Qubit3.0 Flurometer (Invitrogen, USA), libraries were analyzed for size distribution by NGS3K/Caliper and quantified by real-time PCR (3 nM).
Submitted by: Institute of Microbiology, Chinese Academy of Sciences
Study: Novel Spacer Sequence Acquired by CRISPR-cas System of Haloarcula hispanica ATCC33960
PRJNA984824 • SRP444516 • All experiments • All runs
show Abstracthide Abstract
Sequencing analysis of newly acquired spacers detected by PCR after HHPV-2 (MOI~40) virus infestation of Tm and TAdm mutant strains. Also included are new spacers acquired by TAdm that have not been treated with HHPV2.
Sample: creT mut
SAMN35779350 • SRS18004709 • All experiments • All runs
Organism: Haloarcula hispanica
Library:
Name: FDSW220189986-1r
Instrument: Illumina HiSeq 2500
Strategy: WGA
Source: GENOMIC
Selection: other
Layout: SINGLE
Runs: 1 run, 5.3M spots, 1.6G bases, 488.1Mb
Run# of Spots# of BasesSizePublished
SRR250548235,278,8631.6G488.1Mb2023-06-28

ID:
28240443

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